Journal:

Article Title: Multiplex PCR Amplimer Conformation Analysis for Rapid Detection of gyrA Mutations in Fluoroquinolone-Resistant Mycobacterium tuberculosis Clinical Isolates

doi: 10.1128/AAC.48.2.596-601.2004

Figure Lengend Snippet: Relative fluoroquinolone susceptibilities of 32 ofloxacin-resistant M. tuberculosis isolates exhibiting seven gyrA mutation types a

Article Snippet: Relative fluoroquinolone susceptibilities of 32 ofloxacin-resistant M. tuberculosis isolates exhibiting seven gyrA mutation types a table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Amino acid change Relative MIC (fold) Moxifloxacin Gatifloxacin Sitafloxacin Ofloxacin Levofloxacin Sparfloxacin MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 Ala90→Val 4 8 4 8 2 4 32 64 16 32 33 33 Ser91→Pro 4 16 8 16 4 16 16 16 8 8 8 8 Asp94→Gly 8 16 16 16 8 8 64 64 32 32 67 133 Asp94→His 8 8 16 16 8 8 64 64 32 32 67 133 Asp94→Tyr 8 8 8 16 8 16 64 64 32 32 67 133 Asp94→Ala 4 8 8 8 2 2 32 32 16 16 33 33 Asp94→Asn b 8 16 16 32 16 33 Open in a separate window a MICs are given relative to those for the two ATCC strains. b Only one strain harboring the Asp-94-Asn change was detected.

Techniques: Mutagenesis

Journal:

Article Title: Multiplex PCR Amplimer Conformation Analysis for Rapid Detection of gyrA Mutations in Fluoroquinolone-Resistant Mycobacterium tuberculosis Clinical Isolates

doi: 10.1128/AAC.48.2.596-601.2004

Figure Lengend Snippet: MICs relative to control strains a

Article Snippet: Relative fluoroquinolone susceptibilities of 32 ofloxacin-resistant M. tuberculosis isolates exhibiting seven gyrA mutation types a table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Amino acid change Relative MIC (fold) Moxifloxacin Gatifloxacin Sitafloxacin Ofloxacin Levofloxacin Sparfloxacin MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 Ala90→Val 4 8 4 8 2 4 32 64 16 32 33 33 Ser91→Pro 4 16 8 16 4 16 16 16 8 8 8 8 Asp94→Gly 8 16 16 16 8 8 64 64 32 32 67 133 Asp94→His 8 8 16 16 8 8 64 64 32 32 67 133 Asp94→Tyr 8 8 8 16 8 16 64 64 32 32 67 133 Asp94→Ala 4 8 8 8 2 2 32 32 16 16 33 33 Asp94→Asn b 8 16 16 32 16 33 Open in a separate window a MICs are given relative to those for the two ATCC strains. b Only one strain harboring the Asp-94-Asn change was detected.

Techniques: Control

Journal:

Article Title: Multiplex PCR Amplimer Conformation Analysis for Rapid Detection of gyrA Mutations in Fluoroquinolone-Resistant Mycobacterium tuberculosis Clinical Isolates

doi: 10.1128/AAC.48.2.596-601.2004

Figure Lengend Snippet: Single-stranded conformation polymorphism and MPAC patterns of Mycobacterium tuberculosis strains harboring gyrA mutations. (a) Single-stranded conformation polymorphism patterns of PCR products generated with primers 3F/3R. (b) MPAC patterns of multiplex PCR products generated with primers 1F/1R, 2F/2R, and 3F/3R in a single reaction. (c) MPAC patterns of a mixture of two PCR products generated by primers 2F/2R and primers 3F/3R, respectively. A, Strains ATCC 27294 and ATCC 25177; B, no mutation in the region encoding amino acids 90 through 94; C, Ala90→Val; D, Ser91→Pro; E, Asp94→Gly; F, Asp94→His; G, Asp94→Tyr; H, Asp94→Ala; I, Asp94→Asn. Apart from the two ATCC strains, all isolates contained a Ser→Thr substitution at position 95; hence, patterns A and B are different.

Article Snippet: Relative fluoroquinolone susceptibilities of 32 ofloxacin-resistant M. tuberculosis isolates exhibiting seven gyrA mutation types a table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Amino acid change Relative MIC (fold) Moxifloxacin Gatifloxacin Sitafloxacin Ofloxacin Levofloxacin Sparfloxacin MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 MIC 50 MIC 90 Ala90→Val 4 8 4 8 2 4 32 64 16 32 33 33 Ser91→Pro 4 16 8 16 4 16 16 16 8 8 8 8 Asp94→Gly 8 16 16 16 8 8 64 64 32 32 67 133 Asp94→His 8 8 16 16 8 8 64 64 32 32 67 133 Asp94→Tyr 8 8 8 16 8 16 64 64 32 32 67 133 Asp94→Ala 4 8 8 8 2 2 32 32 16 16 33 33 Asp94→Asn b 8 16 16 32 16 33 Open in a separate window a MICs are given relative to those for the two ATCC strains. b Only one strain harboring the Asp-94-Asn change was detected.

Techniques: Generated, Multiplex Assay, Mutagenesis